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DescriptionThis filter generates cracks which you can use as layers for more complex images or brushes. More work needs to be done to improve this filter but some nice patterns are generated.This filter started with the 'Cracks Stencil' filter by ronviers.
DescriptionThis is my Eighty-Second filter. I hope you like it and find it useful. This filter was made possible in part with a group called " Crack subtree " From a filter called " Plasattack " by KGtheway28. Thanx KGtheway28 for your filter.
DescriptionCreates cracked concrete floor. Lighter sun burned outer layer has became to fall off due weather, revealing darker inner structure.Erosion > larger values reveal more inner structure
DescriptionThis filter generate lichen, moss and dirt clad cracked stone surfaces with multi-tile, which can be used to create several textures with different internal look that all tile together.
The Filter Library is a free online repository of filters created by our designers and Filter Forge users. You can submit your own filters to the Filter Library, and if they get popular with the Filter Forge users, you can earn rewards including a free copy of Filter Forge. Well never mind, they all are here for free.
On the surface, Filter Forge is just a Photoshop plugin, a pack of filters that generate textures, create visual effects, enhance photos, process images. However, there are 3 things that make Filter Forge unique:
2. You get free access to 12077 user-created filters. Anyone can contribute their textures and effects to the online filter collection so it grows with every submitted filter. This means the more people use Filter Forge, the better it gets.
Every filter author must have stumbled into this: you create a filter and want to carefully select presets to showcase its usage, but arranging filter presets is pain. With Filter Forge 6 the pain is gone: you can now drag and drop presets to reorder them.
Welcome to Skyforge! We have a small welcome present for all new players joining us from Steam. This Free Steam Welcome Pack contains a permanent class unlock and some Premium Account time to help you start in the world of Aelion.
UNO Synth Pro gives you the power to create nearly any analog synth sound you can imagine. With its unique dual-filter, 3-oscillator paraphonic design, 256 presets, 64-step sequencer, studio-grade effects, expanded connections and much more, UNO Synth Pro breaks new ground for music makers.
Also, filters can generate diffuse, specular, bump, and normalmaps, which makes it an essential tool for artists creating3D models and environments, architectural visualization and high-end game content
PRINSEQ is a tool that generates summary statistics of sequence and quality data and that is used to filter, reformat and trim next-generation sequence data. It is particular designed for 454/Roche data, but can also be used for other types of sequence data. PRINSEQ is available through a user-friendly web interface or as standalone version. The standalone version is primarily designed for data preprocessing and does not generate summary statistics in graphical form.
The interactive web interface provides summary statistics for quality control that can help to choose parameters for data preprocessing. PRINSEQ provides filter, trim and reformat options for data preprocessing.
Check those numbers to make sure it matches approximately the manufacturer estimates. If your numbers are off too much, check the raw data and filter statistics in "454BaseCallerMetrics" and "454QualityFilterMetrics".
Minimum and maximum read lengthSequences in the SFF files can be as short as 40 bp (shorter sequences are filtered during signal processing). For multiplexed samples, the MID trimmed sequences can be as short at 28 bp (assuming a 12 bp MID tag). Such short sequences can cause problems during, for example, database searches to find similar sequences. Short sequences are more likely to match at a random position by chance than longer sequences and may therefore result in false positive functional or taxonomical assignments. Furthermore, short sequences are likely to be quality trimmed during the signal-processing step and of lower quality with possible sequencing errors.In some cases, sequences can be much longer than several standard deviations above the mean length (e.g. 1,500+ bp for a 500 bp mean length with a 100 bp standard deviation). Those sequences should be used with caution as they likely contain long stretches of homopolymer runs as in the following example below. Homopolymers are a known issue of pyrosequencing technologies such as 454/Roche [1].
The GC content distribution of most samples should follow a normal distribution. In some cases, a bi-modal distribution can be observed, especially for metagenomic data sets. The GC content plot in PRINSEQ marks the mean GC content (M) and the GC content for one and two standard deviations (1SD and 2SD). This can help to decide where to set the GC content thresholds, if a GC content filter will be applied. The plot can also be used to find the thresholds or range to select sequences from a bi-modal distribution.
PRINSEQ offers additional plots to investigate the sequence duplicates from different points of view. The plot showing the sequence duplication levels (with number of sequences with one duplicate, two duplicates, three duplicates, ...) can be used to identify the distribution of duplicates (e.g. do many sequences have only a few duplicates). The plot showing the highest number of duplicates for a single sequence (top 100) can help to indentify if only a few sequences have many duplicates (e.g. as a result of specific PCR amplification) and what the highest duplication numbers are.Depending on the dataset and downstream analysis, it should be considered to filter sequence duplicates. The main purpose of removing duplicates is to mitigate the effects of PCR amplification bias introduced during library construction. In addition, removing duplicates can result in computational benefits by reducing the number of sequences that need to be processed and by lowering the memory requirements. Sequence duplicates can also impact abundance or expression measures and can result in false variant (SNP) calling. The example below shows the alignment of sequences against a reference sequence (gray). The sequence duplicates (starting at the same position) suggest a possibly false frequency of base C at the position marked in bold.
The DeconSeq web version allows users to manage the filter, trim and reformat options in different ways. There are features to save or reset the options currently set, to load previously saved options or to select one of the option pre-sets. Loaded options or selected pre-sets will only be applied after clicking on the "Set new options" button at the bottom. This allows users to review the options before actually setting them.
Length relatedSequences in the SFF files can be as short as 40 bp (shorter sequences are filtered during signal processing). For multiplexed samples, the MID trimmed sequences can be as short at 28 bp (assuming a 12 bp MID tag). Such short sequences can cause problems during, for example, database searches to find similar sequences. Short sequences are more likely to match at a random position by chance than longer sequences and may therefore result in false positive functional or taxonomical assignments. Furthermore, short sequences are likely to be quality trimmed during the signal-processing step and of lower quality with possible sequencing errors.
GC content relatedThe GC content distribution of most samples should follow a normal distribution. In some cases, a bi-modal distribution can be observed, especially for metagenomic datasets. This filter is rarely used, but proved useful to separate sequences in a bi-modal distribution.
Data content relatedTo select a subset of all sequence in a dataset, the number of wanted sequences can be specified. The first X sequences passing all other specified filters can be selected this way.
Depending on the dataset and downstream analysis, it should be considered to filter sequence duplicates. The main purpose of removing duplicates is to mitigate the effects of PCR amplification bias introduced during library construction. In addition, removing duplicates can result in computational benefits by reducing the number of sequences that need to be processed and by lowering the memory requirements. Sequence duplicates can also impact abundance or expression measures and can result in false variant (SNP) calling.
PRINSEQ filters duplicates without allowing mismatches, as artificial duplicates tend to have the same errors and error-models are computationally more expensive. Programs such as cdhit-454 [4] use clustering techniques to identify near identical duplicates. However, those methods tend to miss duplicates identified by PRINSEQ due to the used clustering methods. For best results, duplicates should initially be filtered using PRINSEQ and then optionally using clustering methods.
Custom filter parametersThe custom filter allows the specification of user defined filter using a two value system. Each new filter should be defined on a separate line and values should be separated by space. The first value defines the filter pattern (any combination of the letters "ACGTN"). The second value defines the number of repeats or percentage of occurrence of the filter pattern. The percentage values are defined by a number followed by the %-sign (without space). If no %-sign is given, it is assumed that the value specifies the number of repeats of the filter pattern.
Examples: AAT 8 filters out sequences containing AATAATAATAATAATAATAATAAT anywhere in the sequence T 70% filters out sequences with more than 70% T's in the sequence A 15 filters out sequences containing AAAAAAAAAAAAAAA anywhere in the sequence 2b1af7f3a8
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