young justice season 1 720p downloadNitric oxide release from a periplasmic polyphosphate-binding protein in Pseudomonas aeruginosa.
A protein with affinity for polyphosphate was identified in Pseudomonas aeruginosa cells. Electrophoresis of soluble proteins from an anaerobically grown strain under non-reducing conditions showed a band at 21.5 kDa. The amino acid sequence of the polyphosphate-binding protein (PPB) revealed similarity to periplasmic polyphosphate-binding proteins in a number of Gram-negative and Gram-positive organisms. PPB was highly labile in the presence of O2. Addition of the peroxynitrite ion to PPB resulted in conversion to a protein exhibiting the same electrophoretic mobility as nitrite-insensitive PPB, suggesting that nitrite was released from this protein under aqueous conditions. PPB was overproduced and partially purified by ammonium sulfate fractionation and phosphocellulose chromatography. Nitrite released from PPB was detected by the Griess method. PPB was purified to homogeneity by anion exchange and gel filtration chromatography. PPB exhibited cooperative binding to polyphosphate (KD = 7.4 mM). The association rate constant was 3.4 x 10(4) M-1 s-1 and the dissociation rate constant was 0.005 s-1. Protein sequence similarity with many polyphosphate-binding proteins suggests that PPB is a member of a family of proteins with a variety of cellular functions, including nitric oxide generation and signal transduction.Aussa and Komurawa-go
Aussa and Komurawa-go were two distinct entities, but in the Kingdom of Awa, both were regarded as descendants of the same lineage, the royal family of Awa and Azuchi. The name of the territories of Aussa and Komurawa-go sometimes collectively referred to as the Aussa district, which in turn is a part of the ancient province of Awa, currently within the modern prefectures of Ōita and Ehime. The Aussa district was divided into four provinces: Aussa, Komurawa-go, Kaifu, and Masu; in the Edo period, it was ruled by the Ichinose clan.